The brilliance of discovery

Sirigen Inc.

Microscopy & digital pathology

Microscopy

Sirigen’s bright HSF™ polymers are an excellent choice for microscopy, digital pathology and imaging applications.

Sirigen’s polymers offer significant advantages for microscopy and image based applications. The brightness of the reporters offers unparalleled sensitivity and signal to noise ratios compared with conventional fluors. It is often possible to use HSF™ labeled primary antibodies to deliver impressive results without the need for signal amplification through a secondary. This offers users great hands on convenience and time savings along with improved options to use their best antibodies together.

Compared to traditional dyes Sirigen’s HSF™ reporters show marked improvements in photostability showing greater signal after more than 5 scans than the initial signal from a conventional dye.

Confocal microscopy of mouse brain labeled with β-tubulin III neural marker primary Ab and detected with fluor labeled secondary. All scans at equal laser power but increased PMT settings for conventional fluor.

Scan

The Sirigen reporters are compatible with a wide range of techniques and reagents with very little optimization.

False color multicolor staining of wholemount mouse retina with Brilliant Violet 421™ and two Alexa Fluor® labeled secondaries.

2 photon imaging of mouse cortical neurons with Brilliant Violet 421™.

Frozen human colon stained with biotinylatyed anti-desmin primary and detected with Brilliant Violet 421™ streptavidin. Section PI counterstained.

Human leukemic cell line chromosome spread stained with biotinylated FISH probe and Brilliant Violet 421™ streptavidin.

Acknowledgements
Mouse images courtesy of Drs Mary Raven and Geoff Lewis, NRI-MCDB Microscopy Facility at UCSB. Human images courtesy of Dr Larry McIntyre.